Response Surface Methodology for Optimization of Small Peptide Preparation from Paphia undulate Meat by Acid Hydrolysis

In this study,an acid hydrolysis procedure for preparing small peptides from Paphia undulate meat was proposed and optimized using response surface methodology(RSM).A quadratic regression model describing small peptide yield as a function of 3 variables was created based on a 5-level quadratic orthogonal rotation combination design,whose goodness of fit was 92.09%.The optimal hydrolysis conditions were found as follows: raw material-to-water ratio of 1:3,hydrochloric acid concentration of 6.4 mol/L,hydrolysis temperature of 92 ℃ and hydrolysis time of 5.3 h.Under the optimal conditions,the predicted and experimental recovery rates of small peptides were 84.04% and 82.21%,respectively.SDS-PAGE revealed that the smallest peptides in the resulting hydrolysate were less than 2 kD.