Determination of Chlorogenic Acid and Baicalin In Shuanghuanglian Koufuye by HPLC

Aim To develop an HPLC quantitative method for the determination of chlorgenic acid and baicalin in Shuanghuanglian Koufuye.Methods The chromatographic conditions include the column C18(Agilent ZORBAX Eclipse XDB-C18,4.6mm×150mm,5Micron)mobile phase:A methanol-water-phosphorc acid(50∶950∶5),B methanol;gradient elution:0～30,A:95%～35%,B:5%～65%.The flow rate was 1.0ml/min and monitored at 327nm.Results the retention time of chlorgenic acid and baicalin was 10.6min and 24min respectively.The regress equation for chlorgenic acid was Y=0.032 98X-0.093 86,r=0.999 9,and the linear range was 5.040～45.36μg·ml-1.Baicalin was Y=0.051 55X-1.286 4,r=0.999 9 and the linear range was 44.88～403.9μg·ml-1.The average recovery of chlorgenic acid and baicalin was(n=6)99.6% and 100.0%;RSD 0.32% and 1.0% respectively.Conclusion This method conform to the method of determination specified in CP 2005,and was sensiteve,time-saving and accurate.