Substrate Inhibition of Succinic Acid Fermentation with Actinobacillus succinogenes NJ113

Anaerobic fermentative production of succinic acid by Actinobacillus succinogenes NJ113 with glucose as the substrate in batch fermentation was studied.Fed-batch fermentation was performed to minimize the inhibition effect of substrate (glucose).In this case,45.27 g/L succinic acid and productivity of 1.74 g/(L?h) were attained after 26 h incubation with total glucose concentration of 60 g/L using Na2CO3 as the pH regulator,with a level less than 10 g/L of glucose concentration.Compared with the batch fermentation,the fermentation period was already shortened by up to 18.75%.The yield of succinic acid and productivity were raised by 5.44% and 31.82% respectively in fed-batch fermentation,and the by-product formic acid decreased and the acetic acid increased in the process.