Effects of epinephrine in combination with rhG-CSF on mobilizing hematopoietic stem and progenitor cells in mice

Objective To investigate the mobilizing effect of epinephrine on HSPC by comparative study on the effects of epinephrine and recombinant human granulocyte colony stimulating factor(rhG-CSF) when used alone or in sequential combination in mobilizing hematopoietic stem and progenitor cells(HSPCs) in mice and optimize the strategy of mobilization.Methods 48 BALB/c mice were randomly divided into 4 groups,with 12 mice in each group.In Group A(the control group),each mice was given a subcutaneous injection of NS 100 μl·d-1;in Group B,each mice was subcutaneously injected with rhG-CSF 250 μg·kg-1·d-1 alone;in Group C,each mice was intraperitoneally injected with epinephrine 2.5 mg·kg-1·d-1 alone;in Group D,each mice was injected in sequential combination of rhG-CSF 250 μg·kg-1·d-1 subcutaneously and epinephrine 2.5 mg·kg-1·d-1 intraperitoneally 3 h after rhG-CSF.All the groups were injected for five consecutive days.Variations of the white blood cell(WBC) count,and the proportions of KL and KSL cells in peripheral blood of mice were dynamically observed.Results The WBC count,and the proportions of KL and KSL cells in peripheral blood of Group D were all markedly higher than those of Group B(P0.05) and Group C or Group A(P0.01);Group B mobilized more WBC,KL and KSL cells in peripheral blood as compared to those of Group C and Group A(P0.01);Group C had much more yield of WBC than that of Group A(P0.01),while the proportions of KL and KSL cells had no significant difference(P0.05).Conclusion Epinephrine can participate in mobilizing HSPC in coordination with rhG-CSF.