Effects of rhEPO and rhG-CSF on Mobilization of CD90~+ Cells in Mice

Objective To investigate effects of recombinant human erythropoietin(rhEPO) and recombinant human granulocyte colony stimulating factor(rhG-CSF) on mobilization of CD90+ cells in mice,and compare their mobilization efficiency.Methods Thirty male Kunming mice were randomly divided into control,rhG-CSF and rhEPO groups.The mice were subcutaneously injected with rhG-CSF in a dose of 40 μg·kg-1·d-1,rhEPO in a dose of 250 IU·kg-1·d-1 or saline for 5 days,respectively.The peripheral blood were collected at six hours afer the final injection,the number of absolute nuclear cells(ANC) and mononuclear cells(MNC) were counted,the proportion of CD90+,CD34+CD90+ and CD90+CD34-cells in ANC was detected by flow cytometry.Results Compared with control group,the number of ANC and MNC in peripheral blood were increased in both rhG-CSF group and rhEPO group(P0.05),but there was no significant difference between rhG-CSF and rhEPO group(P0.05).The proportion of CD90+ cells in rhEPO group was(56.49±9.06)%,and significantly increased than that of rhG-CSF group(P0.05);while the proportion of CD90+ cells in ANC had no significant difference between rhG-CSF group and control group(P0.05).The proportion of CD90+CD34-in rhEPO group was increased to(53.5±8.9)% and was accoutable for the majority of CD90+ cells.Conclusion Both rhG-CSF and rhEPO have a strong ability of hematopoietic stem cells mobilization,the mobilization effect of rhEPO was more greater than rhG-CSF in CD90+ cells mobilization,which suggests that rhEPO could mobilize primitive hematopoietic stem cells more effectively.