Comparative study on the effects of G-CSF and GM-CSF on mobilization of peripheral blood stem cells in mice

Objective To optimize the regime for mobilizing peripheral blood stem cells in mice by comparing the effects of recombinant human granulocyte colony stimulating factor(rhG-CSF) and recombinant human granulocyte-macrophage colony stimulating factors(rhGM-CSF) when used alone or in concurrent or sequential combination.Methods 72 BALB/c mice were randomly divided into 6 groups,12 mice in each group.The experiment groups were assigned sequentially to the following 5-day regimens for mobilization: rhG-CSF 400 μg·kg-1·d-1 alone;rhGM-CSF 400 μg·kg-1·d-1 alone;concurrent combination of rhG-CSF 200 μg·kg-1·d-1 and rhGM-CSF 200 μg·kg-1·d-1;sequential combination of rhGM-CSF 400 μg·kg-1·d-1 for 3 days and rhG-CSF 400 μg·kg-1·d-1 for 2 days;sequential combination of rhG-CSF 400 μg·kg-1·d-1 for 3 days and rhGM-CSF 400 μg·kg-1·d-1 for 2 days.The control group was given a subcutaneous injection of saline 0.2 ml qd for 5 days.The white blood cell(WBC) count,the percentage of CD34+ cells and the number of CFU-GM in peripheral blood of mice were dynamically observed.Results The WBC,CD34+ cells,CD34+CD38-cells and CFU-GM yield in peripheral blood of the experimental groups were all higher than that of the control group(P0.01).The experimental groups treated with rhG-CSF alone or in combination with rhGM-CSF resulted in increase in number of WBC and CD34+ cells in peripheral blood as compared to that of using rhGM-CSF alone(P0.01).The number of CD34+CD38-cell of the group treated with rhG-CSF alone was lower than that of the other experimental groups(P0.01).The yield of CFU-GM was higher in the combination groups than in the single G-CSF and single rhGM-CSF groups(P0.01).Conclusions The combined use of G-CSF and GM-CSF is a better alternative for peripheral blood stem cell mobilization.