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2009•Heilongjiang yiyao kexueRequires access

Determination of quercetin and kaempferol in Herba Lysimachiae Granules by HPLC

Sun Qi-fen

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Abstract

Objective:To establish a method for the determination of quercetin and kaempferol in Herba Lysimachiae Granules by HPLC.Methods:AglientC18(150mm×4.6mm,5μm)column was used.The mobile phase was methanol-water-phosphoric acid solution(500:500:1).The flow rate was 10mL/min.The detection wavelength was 360 nm and the column temperature was 30℃.Results:The linear relation was good.The average recovery of quercetin was 95.42% with RSD=3.11%;The average recovery of kaemferol was 100.5% with RSD=0.978%.Conclusion:The method is simple,accurate and has good reproducibility.It can be used for the quality control of the granules.

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What this paper is about

Objective:To establish a method for the determination of quercetin and kaempferol in Herba Lysimachiae Granules by HPLC.Methods:AglientC18(150mm×4.6mm,5μm)column was used.The mobile phase was methanol-water-phosphoric acid solution(500:500:1).The flow rate was 10mL/min.The detection wavelength was 360 nm and the column temperature was 30℃.Results:The linear relation was good.The average recovery of quercetin was 95.42% with RSD=3.11%;The average recovery of kaemferol was 100.5% with RSD=0.978%.Conclusion:The method is simple,accurate and has good reproducibility.It can be used for the quality control of the granules.

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Available abstract

Objective:To establish a method for the determination of quercetin and kaempferol in Herba Lysimachiae Granules by HPLC.Methods:AglientC18(150mm×4.6mm,5μm)column was used.The mobile phase was methanol-water-phosphoric acid solution(500:500:1).The flow rate was 10mL/min.The detection wavelength was 360 nm and the column temperature was 30℃.Results:The linear relation was good.The average recovery of quercetin was 95.42% with RSD=3.11%;The average recovery of kaemferol was 100.5% with RSD=0.978%.Conclusion:The method is simple,accurate and has good reproducibility.It can be used for the quality control of the granules.

Key concepts: Kaempferol, Quercetin, Phosphoric acid, Chromatography, Chemistry, High-performance liquid chromatography, Reproducibility, Biochemistry

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