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2009•Zhonghua zhongyiyao xuekanRequires access

Determination of Quercetin and Kaempferol in Herba Lysimachiae by HPLC

Ying Wang

Open publisher page 1 citations

Abstract

Objective:To determine the content of quercetin and kaempferol in Herba Lysimachiae by HPLC.Methods:Diamond ODS column was used.Mobile phase consisted of methanol-0.45% phosphate.UV detector wave length was set at 366nm.Results:The linearity was from 0.04724 to 0.2362μg for quercetin and from 0.1424 to 0.4272μg for kaempferol,and the average recovery was 99.8% for quercetin and 99.9% for kaempferol with RSD 1.39% and 1.48% respectively.Conclusion:The method is sensitive and accurate,and can be used for the quality control of Herba Lysimachiae.

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Objective:To determine the content of quercetin and kaempferol in Herba Lysimachiae by HPLC.Methods:Diamond ODS column was used.Mobile phase consisted of methanol-0.45% phosphate.UV detector wave length was set at 366nm.Results:The linearity was from 0.04724 to 0.2362μg for quercetin and from 0.1424 to 0.4272μg for kaempferol,and the average recovery was 99.8% for quercetin and 99.9% for kaempferol with RSD 1.39% and 1.48% respectively.Conclusion:The method is sensitive and accurate,and can be used for the quality control of Herba Lysimachiae.

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Available abstract

Objective:To determine the content of quercetin and kaempferol in Herba Lysimachiae by HPLC.Methods:Diamond ODS column was used.Mobile phase consisted of methanol-0.45% phosphate.UV detector wave length was set at 366nm.Results:The linearity was from 0.04724 to 0.2362μg for quercetin and from 0.1424 to 0.4272μg for kaempferol,and the average recovery was 99.8% for quercetin and 99.9% for kaempferol with RSD 1.39% and 1.48% respectively.Conclusion:The method is sensitive and accurate,and can be used for the quality control of Herba Lysimachiae.

Key concepts: Kaempferol, Quercetin, High-performance liquid chromatography, Chemistry, Chromatography, Methanol, Traditional medicine, Medicine

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