Determination of Quercetin and Kaempferol in Herba Lysimachiae Granules by HPLC

ObjectiveTo establish an HPLC method for the determination of quercetin and kaempferol in Herba Lysimachiae Granules.MethodsDalian Elite Hypersil ODS2 5μm(200 mm×4.6 mm)column was used.The mobile phase was methanol-(0.4)% phosphoric acid solution(45∶55).The flow rate was 1.0 ml·min~(-1);The detection wavelength was 360 nm;The column temperature was 30℃.ResultsThe linear relation was good.The average recovery of quercetin was 101.2% with RSD=(1.14)%;The average recovery of kaemferol was 100.1% with RSDwas(1.33)%.ConclusionThe method is simple,accurate and with a good reproducibility and can be used for the quality control of the granules.