2011•Chinese Journal of Clinical Rational Drug UseRequires access

Determination of ginsenoside Rg1 and ginsenoside Re in Renshenbeiqi capsule by HPLC

YE Yu-hu

Abstract

Objective To establish HPLC method for the content determination of ginsenoside Rg1 and ginsenoside Re in Renshenbeiqi tablet.Methods A ODS 2HYPERSIL colum(250mm×4.6mm 5μm)was used,and acetonitrile-0.1% phosphoric acid (20∶80) as the mobile phase;The detection wavelength was at 203 nm.The flow rate was 1.0ml/min.Results The calibration curves of ginsenoside Rg1 and ginsenoside Re were linear in the range 0.2024μg～5.0600μg (r=0.9995) and 0.2152μg～5.3800μg (r=0.9998),respectively.The average recoveries were 99.0%,(RSD=1.04%,n=9)for ginsenoside Rg1 and 98.2%(RSD=0.70%,(n=9)for ginsenoside Re.Conclusion HPLC can determine the content of ginsenoside Rg1 and ginsenoside Re in Renshenbeiqi capsule simply,rapidly and accurately.

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Key concepts: Ginsenoside Rg1, Medicine, Chromatography, High-performance liquid chromatography, Capsule, Ginsenoside, Phosphoric acid, Calibration curve