Analysis of several physiological and biochemical index induced by Sclerotinia sclerotiorum among different non-heading Chinese cabbages

Non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) varieties(003-R-6,Y3-R-4,109-S-9 and 003-S-7) with different resistance to Sclerotinia sclerotiorum were selected as materials.At seedling stage,leaf agar disk inoculation method was used to test resistance to Sclerotinia sclerotiorum.Leaves were used to analyze the physiological and biochemical changes such as the content of malondialdehyde(MDA),free amino acid(FAA),the activity of chitinase and β-1,3-glucanase.The results showed that leaf agar disk inoculation method was highly correlated with field induction,and it could well represent the resistance and susceptibility of the tested varieties.In addition,physiological and biochemical changes were observed after the inoculation.The increase of MDA,FAA in the susceptible variety 003-S-7 was higher than that of resistant variety 003-R-6.On the contrary,the increase of chitinase and β-1,3-glucanase activity in the resistant varieties was higher than that of the susceptible ones.The results also indicated that change of the content or activity of the physiological and biochemical characters after inoculation is correlated with Sclerotinia sclerotiorum resistance to some extent.