2011•Shenyang Nongye Daxue xuebaoRequires access
Techniques for Isolated Microspore Culture in Yellow Heading Spring Chinese Cabbage
Hui Feng
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Abstract
Isolated microspore culture was carried out with 12 F1 yellow Chinese cabbage introduced from South Korea and Japan.Frequencies of embryo-genesis,factors influencing plantlet regeneration were studied.The results showed that genotypes had very significant effect on frequency of embryogenesis.The reaction of genotypes to growth regulators added in medium was different.Low temperature and hermetic sealing could promote the development of embryoids.The optimal medium for rooting was MS +0.1mg·L-1 NAA.An efficient culture system for yellow heading spring Chinese cabbage was established through the optimization of culture conditions,and totally 445 regenerated plants of 11 hybrid cultivar were obtained,in which 362 were identified as double haploid.