Determination the concentration of domperidone in human plasma by LC-MS/MS

Objective To develop a LC-MS/MS method for the determination the concentration of the domperidone in human plasma.Methods Domperidone and the internal standard were treated by methanol precipitation to remove protein component from human plasma,and then separated on an Agilent TC-C18 column with gradient elution.The mobile phase consisted of methanol(containing 0.025% formic acid)-1 mmol·L-1 ammonium formate solution(containing 0.05% formic acid).The flow rate was 1.0 mL·min-1.Detection was carried out by multiple reaction monitoring on LC-MS/MS system.Results The assay was linear over the range 0.1-50.0 ng·mL-1.Intra-and inter-day precision were both less than 15%,respectively.The recoveries of domperidone were more than 80%.Conclusion It is a rapid,sensitive,selective and reliable method.The assay can be applied for the determination of domperidone in human plasma and the study on pharmacokinetics.