Decolorization of dyes by Phanerochaete chrysosporium and Trametes versicolor growing on adsorptive carriers

Phanerochaete chrysosporium and Trametes versicolor were cultured on different carriers for continuous decolorization,the performance of different Phanerochaete chrysosporium and Trametes versicolor were researched and compared.In this paper,Phanerochaete chrysosporium and Trametes versicolor were cultured in liquid containing spherical sawdust,corncob and peanut shells under oscillation conditions.During few days of cultivation,large amount of bacteria was grew and attached on the carrier surface in membranous and lump state.After consecutive 4 rounds of decolorization,Phanerochaete chrysosporium and Trametes versicolor growing on sawdust carrier had significant advantage both in continuous decolorization and producing enzymes,so the optimal adsorptive carrier was sawdust.After consecutive 2 rounds and 12 d of reactive black RB5 decolorization,the Phanerochaete chrysosporium culture with sawdust as adsorptive carrier could achieve the decolorization of 97%;after three rounds of dye supplemented,the cultures also could remove nearly 96% of M-3BE and the maximum production of manganese dependant peroxidase enzyme(MnP) and lignin peroxidase enzyme(LiP) was 611,1 477 U/L.In the actual application,Phanerochaete chrysosporium and Trametes versicolor culture with sawdust as carriers should be added to dye wastewater treatment system to strengthen the biological treatment effect.