2005•Journal of Hebei UniversityRequires access

A Modified Alkaline Lysis Method for Extracting Plasmid DNA

Zhou Xin

Abstract

The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. The paper improved the method by putting solution Ⅰ,Ⅱ,Ⅲ one by one in the reaction system with the bacteria amount under control. The improved procedure, named a modified method, predigested steps and saved experimental time, while the quantity and the quality of plasmid DNA were maintained.

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Available abstract

Key concepts: Alkaline lysis, Plasmid, Lysis, DNA, Plasmid preparation, Bacteria, Chemistry, Chromatography