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2008•Journal of Nanhua UniversityRequires access

DPI Inhibiting Apoptosis and NADPH Oxidase 4 Expression of hUVECs Induced by AngII

Sun Wen-qing

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Abstract

Objective To observe the influence of DPI on apoptosis and NOX4 mRNA expression in hUVECs stimulated by AngⅡ. Methods Morphology of hUVECs was observed by inverted microscope.hUVECs were divided into three groups:control group(treated without AngⅡ for 16 hours),AngⅡ(treated with 10-7 mol/L AngⅡ for 16 hours) and DPI intervention group(treated with 10-7 mol/L AngⅡplus 10μmol/l DPI for 16 hours).mRNA expression of NOX4 was detected by RT-PCR.Cell apoptosis was observed by Hoechest staining. Results The results showed that apoptosis of hUVECs in AngⅡ group was more than that in DPI intervention group.mRNA expression of NOX4 was increased in hUVECs treated with AngⅡ(P0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in hUVECs decreased(P0.05,vs control group,P0.05,vs AngⅡgroup). Conclusion DPI inhibits apoptosis and NADPH oxidase 4 expression of hUVECs induced by AngⅡ.

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Objective To observe the influence of DPI on apoptosis and NOX4 mRNA expression in hUVECs stimulated by AngⅡ. Methods Morphology of hUVECs was observed by inverted microscope.hUVECs were divided into three groups:control group(treated without AngⅡ for 16 hours),AngⅡ(treated with 10-7 mol/L AngⅡ for 16 hours) and DPI intervention group(treated with 10-7 mol/L AngⅡplus 10μmol/l DPI for 16 hours).mRNA expression of NOX4 was detected by RT-PCR.Cell apoptosis was observed by Hoechest staining. Results The results showed that apoptosis of hUVECs in AngⅡ group was more than that in DPI intervention group.mRNA expression of NOX4 was increased in hUVECs treated with AngⅡ(P0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in hUVECs decreased(P0.05,vs control group,P0.05,vs AngⅡgroup). Conclusion DPI inhibits apoptosis and NADPH oxidase 4 expression of hUVECs induced by AngⅡ.

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Available abstract

Objective To observe the influence of DPI on apoptosis and NOX4 mRNA expression in hUVECs stimulated by AngⅡ. Methods Morphology of hUVECs was observed by inverted microscope.hUVECs were divided into three groups:control group(treated without AngⅡ for 16 hours),AngⅡ(treated with 10-7 mol/L AngⅡ for 16 hours) and DPI intervention group(treated with 10-7 mol/L AngⅡplus 10μmol/l DPI for 16 hours).mRNA expression of NOX4 was detected by RT-PCR.Cell apoptosis was observed by Hoechest staining. Results The results showed that apoptosis of hUVECs in AngⅡ group was more than that in DPI intervention group.mRNA expression of NOX4 was increased in hUVECs treated with AngⅡ(P0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in hUVECs decreased(P0.05,vs control group,P0.05,vs AngⅡgroup). Conclusion DPI inhibits apoptosis and NADPH oxidase 4 expression of hUVECs induced by AngⅡ.

Key concepts: NOX4, Apoptosis, NADPH oxidase, Messenger RNA, Chemistry, Molecular biology, Andrology, Oxidative stress

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