Study of protective effect of erigeron injection on renal ischemia-reperfusion injury

Objective To investigate the protective effect of breviscapine on ischemia-reperfusion renal injury,which provides scientific theoretical basis for clinical prevention and treatment of renal ischemia-reperfusion injury.Method 36 SPF male healthy SD rats were randomly divided into 3 groups,12 rats in each group.Group A was ischemia-reperfusion group,group B was erigeron breviscapus injection preconditioning group,and group C was sham operation group.Rats in group A and C were injected with normal saline,while rats in group B were given 12 ml/kg erigeron breviscapus injection by intraperitoneal injection.,After 14 days,renal function,renal antioxidant indexes,renal cell apoptosis indexes and expression of Bcl-2,Bax in renal tissue of three groups were compared.Results The indexes of renal function showed that blood urea nitrogen(BUN) and Serum creatinine(SCr) in group B were significantly lower than group A(P0.05),but significantly higher than group C(P0.05).Renal antioxidant indexes showed that superoxide dismutase(SOD) and malondialdehyde(MDA) in group B were significantly higher than group A(P0.05),but significantly lower than group C(P0.05).The index of renal cell apoptosis showed that the apoptosis index AI in group B was significantly lower than group A(P0.01),but significantly higher than group C(P0.01).The results of immunohistochemical staining showed that the expression of Bcl-2 in group B were significantly higher than that of group A and group C(P0.05),and the expression of Bax in group B was significantly lower than that in group A(P0.05) while significantly higher than group C(F0.01).The ratio of Bcl-2/Bax in group B was significantly higher than group A and group C(P0.05).Conclusion Erigeron Breviscapus Injection may have a protective role on renal ischemia reperfusion injury though antioxidant and anti-apoptosis.