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2002•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of the Flavonoids form Ginkgo Biloba Extact by HPLC with lnternal Standard

Fang Zi

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Abstract

Aim To establish a HPLC method with internal standard for determining the flavonoids found in the extracts of the leaves of ginkgo biloba L.(EGB).Methods Salicylic acid was used as an internal standard.Detection was at 254nm.Amixture of methanol and 0.4% phosphoric acid(1∶1) was usedas mobile phase. Results It was linear in the range of 2～40μg·ml -1 with good correlation (r=0.999 97).Average recovery was101.9%(RSD=3.23% n=3).Conclusion The method is sensitive,simple and accurate.

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What this paper is about

Aim To establish a HPLC method with internal standard for determining the flavonoids found in the extracts of the leaves of ginkgo biloba L.(EGB).Methods Salicylic acid was used as an internal standard.Detection was at 254nm.Amixture of methanol and 0.4% phosphoric acid(1∶1) was usedas mobile phase. Results It was linear in the range of 2～40μg·ml -1 with good correlation (r=0.999 97).Average recovery was101.9%(RSD=3.23% n=3).Conclusion The method is sensitive,simple and accurate.

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Available abstract

Aim To establish a HPLC method with internal standard for determining the flavonoids found in the extracts of the leaves of ginkgo biloba L.(EGB).Methods Salicylic acid was used as an internal standard.Detection was at 254nm.Amixture of methanol and 0.4% phosphoric acid(1∶1) was usedas mobile phase. Results It was linear in the range of 2～40μg·ml -1 with good correlation (r=0.999 97).Average recovery was101.9%(RSD=3.23% n=3).Conclusion The method is sensitive,simple and accurate.

Key concepts: Ginkgo biloba, Chromatography, High-performance liquid chromatography, Phosphoric acid, Chemistry, Ginkgo, Salicylic acid, Methanol

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