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Determination of Total Flavonoids in Ginkgo biloba Health Products by HPLC

Jing Li

Abstract

A more applicable method was developed to determine the total flavonoids in Ginkgo biloba health products by HPLC in this study.The effects of the concentration of HCl,temperature and hydrolysis time on the extraction yield of total flavonoids from Ginkgo biloba health products were studied by response surface methodology(RSM).The samples were determined by HPLC with gradient elution.The optimized condition for hydrolysis was:114 min,79 ℃ and 3.4 mol/L of HCl.This optimum condition led to the flavonoid yield of 8851.468mg/100g.This method showed good linearity and recovery recoveries(95.04%-104.56%) as well as low RSDs(2.0%).This simple,rapid,reproducible method is regarded to be very useful for evaluating the quality of the total flavanoids in Ginkgo biloba health products.

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Available abstract

Key concepts: Ginkgo biloba, Chromatography, Chemistry, High-performance liquid chromatography, Ginkgo, Extraction (chemistry), Flavonoid, Response surface methodology