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2007•Chinese Journal of Hospital PharmacyRequires access

The inhibitory effect of compound Liuyuexue on HBsAg and HBeAg in the HepG2.2.15 cells

Lin Jun

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Abstract

OBJECTIVE To study the inhibitory effect of traditional Chinese medicine compound Liuyuexue (CLYX) on HBV in vitro.METHODS The TC50 and TC0 of CLYX to the HepG2.2.15 cells were determined by MTT assay. At non-cellulotoxic concentration, the different concentrations of CLYX were added respectively to HepG2.2.15 cells, which were incubated subsequently. At 72 h and 144 h after treatment with drugs, the culture media were collected for determining the levels of HBsAg and HBeAg by ELISA.RESULTS TC50=3.070 g·L-1, TC0=0.945 g·L-1. CLYX had little cellulotoxicity. CLYX at the largest non-cellulotoxic concentration could suppress HBsAg and HBeAg expression in HepG2.2.15 cell, and the therapeutic index (TI) 2. CLYX is an effective and low toxic anti-HBV drug.CONCLUSION CLYX can inhibit significantly HBV in vitro, and is a lower toxic drug.

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OBJECTIVE To study the inhibitory effect of traditional Chinese medicine compound Liuyuexue (CLYX) on HBV in vitro.METHODS The TC50 and TC0 of CLYX to the HepG2.2.15 cells were determined by MTT assay. At non-cellulotoxic concentration, the different concentrations of CLYX were added respectively to HepG2.2.15 cells, which were incubated subsequently. At 72 h and 144 h after treatment with drugs, the culture media were collected for determining the levels of HBsAg and HBeAg by ELISA.RESULTS TC50=3.070 g·L-1, TC0=0.945 g·L-1. CLYX had little cellulotoxicity. CLYX at the largest non-cellulotoxic concentration could suppress HBsAg and HBeAg expression in HepG2.2.15 cell, and the therapeutic index (TI) 2. CLYX is an effective and low toxic anti-HBV drug.CONCLUSION CLYX can inhibit significantly HBV in vitro, and is a lower toxic drug.

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Available abstract

OBJECTIVE To study the inhibitory effect of traditional Chinese medicine compound Liuyuexue (CLYX) on HBV in vitro.METHODS The TC50 and TC0 of CLYX to the HepG2.2.15 cells were determined by MTT assay. At non-cellulotoxic concentration, the different concentrations of CLYX were added respectively to HepG2.2.15 cells, which were incubated subsequently. At 72 h and 144 h after treatment with drugs, the culture media were collected for determining the levels of HBsAg and HBeAg by ELISA.RESULTS TC50=3.070 g·L-1, TC0=0.945 g·L-1. CLYX had little cellulotoxicity. CLYX at the largest non-cellulotoxic concentration could suppress HBsAg and HBeAg expression in HepG2.2.15 cell, and the therapeutic index (TI) 2. CLYX is an effective and low toxic anti-HBV drug.CONCLUSION CLYX can inhibit significantly HBV in vitro, and is a lower toxic drug.

Key concepts: HBeAg, HBsAg, In vitro, MTT assay, Pharmacology, Cytotoxicity, IC50, Chemistry

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