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Cloning,Sequence Analysis and Chromosome Mapping of Porcine New NPM1 Gene

Kui Li

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Abstract

The cDNA and fourth intron sequences of porcine NPM1 gene were cloned,and verified by bioinformatics method.The cDNA fragment was 1 195 bp with an entire open reading frame(ORF),encoding 294 amino acids.The intron fragment was 300 bp and splice junctions follow 'GT/AG' rule.The analysis of sequence revealed that the porcine NPM1 gene was highly homologous with that of other species such as human(95%),mouse(91%) and rattus norvegicus(91%) in amino acid.The protein encoded by porcine NPM1 gene had a conserved domain 'Nucleoplasmin'.This gene was assigned to SSC16q21 region and closely linked to SW977 with the LOD of 9.16 by SCHP and IMpRH mapping,respectively.

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What this paper is about

The cDNA and fourth intron sequences of porcine NPM1 gene were cloned,and verified by bioinformatics method.The cDNA fragment was 1 195 bp with an entire open reading frame(ORF),encoding 294 amino acids.The intron fragment was 300 bp and splice junctions follow 'GT/AG' rule.The analysis of sequence revealed that the porcine NPM1 gene was highly homologous with that of other species such as human(95%),mouse(91%) and rattus norvegicus(91%) in amino acid.The protein encoded by porcine NPM1 gene had a conserved domain 'Nucleoplasmin'.This gene was assigned to SSC16q21 region and closely linked to SW977 with the LOD of 9.16 by SCHP and IMpRH mapping,respectively.

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Available abstract

The cDNA and fourth intron sequences of porcine NPM1 gene were cloned,and verified by bioinformatics method.The cDNA fragment was 1 195 bp with an entire open reading frame(ORF),encoding 294 amino acids.The intron fragment was 300 bp and splice junctions follow 'GT/AG' rule.The analysis of sequence revealed that the porcine NPM1 gene was highly homologous with that of other species such as human(95%),mouse(91%) and rattus norvegicus(91%) in amino acid.The protein encoded by porcine NPM1 gene had a conserved domain 'Nucleoplasmin'.This gene was assigned to SSC16q21 region and closely linked to SW977 with the LOD of 9.16 by SCHP and IMpRH mapping,respectively.

Key concepts: Gene, Complementary DNA, Biology, Genetics, Intron, Open reading frame, Molecular biology, Sequence analysis

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Cloning,Sequence Analysis and Chromosome Mapping of Porcine New NPM1 Gene — Research Paper | ScholarLens