Study on Preparation of Concentrated Protein from Double-low Rape Seed

[Objective]The research aimed to find more better methods for the preparation of rape seed concentrated protein.[Method] The L16(45) orthogonal design was used to research the effects of alcohol solution concentration,pH value,liquid-solid ratio,the number of extraction,the time of extraction on the extraction of rape seed concentrated protein.[Result]The results showed that the optimum conditions were pH value 4.5 70% alcohol water solution as extraction solvent,extraction temperature 55℃,with ratio of liquid to solid as 8∶1,washing for seven times and 20 min each time.[Conclusion]By this way,the ratio of rape seed protein reached 64.60%,the content of phytic acid was 2.16% and the content of tannin was 0.