Research on Extraction Technics of Protein in Northeast Rape Leaves

[Objective]Different extraction conditions which influenced extraction percentage of Northeast Rape leaves was studied to ascertain the best extraction technics of protein in Northeast Rape leaves.[Method] Northeast Rape leaves(dry) as subject,using the Kjeldahl Nitrogen Determination method,Soxhlet extraction method and direct drying method,the content of leaf protein,crude fat and water in Northeast Rape leaves were determined to study the influence of different pH,extraction temperature,time and the ratio of solid-liquor on the extraction percentage of leaf protein.[Result] When pH value was 9,temperature was 45 ℃,ratio of solid-liquid was 1∶30,and lixiviation time was 1 hour,the extraction percentage of protein reached 88.20%.[Conclusion] The best technics of leaves protein of Northeast Rape was determined.Scientific basis was provided to explore local rape natural leaf protein production.