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Polymorphism on Intron 2 of Growth Hormone Gene in Goose

Guohong Chen

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Abstract

This study investigated single nucleotide polymorphism(SNP) of growth hormone(GH) gene in 4 local goose breeds(Zi goose,Wanxi White goose,Zhedong white goose and Shitou goose) by PCR-SSCP method. 4 mutations were found among four breeds,which were 360(A/G),474(C/G),551(T/C) and 554(C/T),and there were three kinds of genotypes(EE,EF and FF). The results of χ2 test showed that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in 4 breeds. The genotype frequency of Zi goose showed significant difference with Zhedong white goose and Shitou goose,and the genotype frequency of Wanxi white goose was significant difference with Shitou goose.

About this research paper

What this paper is about

This study investigated single nucleotide polymorphism(SNP) of growth hormone(GH) gene in 4 local goose breeds(Zi goose,Wanxi White goose,Zhedong white goose and Shitou goose) by PCR-SSCP method. 4 mutations were found among four breeds,which were 360(A/G),474(C/G),551(T/C) and 554(C/T),and there were three kinds of genotypes(EE,EF and FF). The results of χ2 test showed that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in 4 breeds. The genotype frequency of Zi goose showed significant difference with Zhedong white goose and Shitou goose,and the genotype frequency of Wanxi white goose was significant difference with Shitou goose.

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Available abstract

This study investigated single nucleotide polymorphism(SNP) of growth hormone(GH) gene in 4 local goose breeds(Zi goose,Wanxi White goose,Zhedong white goose and Shitou goose) by PCR-SSCP method. 4 mutations were found among four breeds,which were 360(A/G),474(C/G),551(T/C) and 554(C/T),and there were three kinds of genotypes(EE,EF and FF). The results of χ2 test showed that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in 4 breeds. The genotype frequency of Zi goose showed significant difference with Zhedong white goose and Shitou goose,and the genotype frequency of Wanxi white goose was significant difference with Shitou goose.

Key concepts: Goose, Biology, White (mutation), Genotype, Genetics, Polymorphism (computer science), Single-nucleotide polymorphism, Gene

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