Study on extraction conditions and structure identification of total flavonoids in tea(Camellia Sinensis)seed

Extraction conditions of total flavonoids in tea seed were researched.Based on the results of single factor experiments,the effect of liquid/material ratio,ethanol concentration,extraction temperature on the extraction yield of total flavonoids was explored to optimize the extraction conditions through central composite design and response surface methodology(RSM).The optimum extraction conditions were determined as follows:liquid/material ratio 26∶1(mL∶g),ethanol concentration 58%,extraction temperature 82℃ and extraction duration of 2h.Under the optimized conditions,the extraction yield of total flavonoids in tea seed was 11.174mg/g.The structure of flavonoids in tea seed which was determined by means of color reaction and UV spectrum was flavanones.