Research on Tissue Culture and Rapid Propagation of Phalaenopsis amabilis

[Objective] The aim was to research the tissue culture and rapid propagation of Phalaenopsis amabilis,and provide reference for batch production of Phalaenopsis amabilis.[Method] Taking tender leaves,the shoot-tips and pedicel lateral buds as explants,the conditions of original corm induction,proliferation and rooting of Phalaenopsis amabilis were discussed.[Result] The result showed that the optimum medium for inducing the tender protocorm of Phalaenopsis amabilis was MS +2.0 mg/L 6-BA +1.0 mg/L NAA +200 ml/L coconut milk,and the inducement rate of shoot-tip was 81.37%.The optimum medium for protocorm proliferation of Phalaenopsis amabilis was MS +1.0 mg/L NAA +2.0 mg/L 6-BA,and the proliferation coefficient was 9.62.The optimum medium for rooting of Phalaenopsis amabilis was MS,the rooting rate was 94.42% and the root growth was fastest and most regular.[Conclusion] In the production,the rapid propagation of Phalaenopsis amabilis should be made by using the rooting subculture method of test-tube seedlings.