2007•WeichangbingxueRequires access
Expression and its Clinical Significance of BRCAA1 Protein in Gastric Cancer Tissue and Various Differentiated Cell Lines
Lizhou Sun
Open publisher page 2 citations
Abstract
Background:BRCAA1 is a novel protein and its role in development of gastric cancer is not yet clear.Aims:To investigate expression and its clinical significance of BRCAA1 protein in gastric cancer tissue and various differentiated cell lines.Methods:Sixty patients with gastric adenocarcinoma were involved,cancerous and paracancerous tissues were taken.The expression of BRCAA1 was detected by immunohistochemical SP staining method.The correlation between expression of BRCAA1 and pathological grading of gastric cancers was analyzed.Western blot was used to analyze the expression of BRCAA1 protein in well differentiated(MKN-1 and MKN-74),moderately differentiated(SGC-7901) and poorly differentiated(KATO-Ⅲand MGC80-3)cell lines.Results:The positivity rate of BRCAA1 protein expression in gastric cancerous tissue(23/60,38.3%)was significantly higher than that in paracancerous tissue(0/60,0%)(P0.01). There were significant differences of BRCAA1 protein expression in well differentiated (2/17,11.8%),moderately differentiated(11/26,42.3%)and poorly differentiated gastric cancer tissues(10/17,58.8%)(P0.05).Western blot analysis showed that expression of BRCAA1 protein was gradually increased with the cell line differentiation degrees decreased. Conclusions:BRCAA1 protein is involved in the development of gastric cancer,and its expression is closely associated with poorly differentiated gastric cancer.BRCAA1 may be a poor prognostic biomarker of gastric cancer.