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[Extract berberine hydrochloride by aqueous two-phase system].

Wen Chi-fu, Xiao-Qi Xiang, Wei-Xiang Zeng, Ting Wu, Chunlian Tian

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Abstract

OBJECTIVE: To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride. METHODS: Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out. RESULTS: 10 mL 95% ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22% into it, regulated its pH to 4, then the system was put into water-bath heater (70 degrees C) for 30 min, the extraction rate could reach 99.29%; Collected the extraction liquid, dried it under 40 degrees C, the purity of berberine hydrochloride was 88.43%. CONCLUSION: This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.

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What this paper is about

OBJECTIVE: To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride. METHODS: Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out. RESULTS: 10 mL 95% ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22% into it, regulated its pH to 4, then the system was put into water-bath heater (70 degrees C) for 30 min, the extraction rate could reach 99.29%; Collected the extraction liquid, dried it under 40 degrees C, the purity of berberine hydrochloride was 88.43%. CONCLUSION: This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.

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Available abstract

OBJECTIVE: To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride. METHODS: Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out. RESULTS: 10 mL 95% ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22% into it, regulated its pH to 4, then the system was put into water-bath heater (70 degrees C) for 30 min, the extraction rate could reach 99.29%; Collected the extraction liquid, dried it under 40 degrees C, the purity of berberine hydrochloride was 88.43%. CONCLUSION: This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.

Key concepts: Berberine hydrochloride, Chromatography, Extraction (chemistry), Aqueous solution, Chemistry, Aqueous two-phase system, Ammonium sulfate, Hydrochloride

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