Expression of E_2 gene of classical swine fever virus in insect cell

The E_2 gene of Classical Swine Fever Virus(CSFV)was subcloned into baculovirus transfer vector and recombinant baculovirus vector pFBHT-E_2 was extracted.Then it was transferred into E.coli DH10Bac and recombinant bacmid was constructed.The recombinant baculovirus was obtained after the tranferation of the recombinant bacmid into sf_9 cell,named as rvBac-E_2.The techniques of SDS-PAGE,Westernblot and ELISA were used to detect and identify the products expressed in sf_9 cell by recombinant baculovirus.The results indicated the E_2 gene was properly expressed in sf_9 cell,and the expressed protein could be recognized by the positive serum of CSFV.