Fusion Expression of GFP and HCLV E2 Gene in Baculovirus System

The envelope glycoprotein E2 of classical swine fever virus(CSFV) was a protective antigen inducing neutral antibody.In order to get soluble recombinant E2 glycoprotein and inspect the protein expression dynamics convinently,the enhanced green fluorescence protein(EGFP) and hog cholera lapinised virus(HCLV) E2 gene were cloned into baculovirus transfer vector pBlueBacHis2A and the recombinant plasmid pBGFPTE2 was constructed.After co-transfection pBGFPTE2 and linear baculovirus Bac-N-Blue into sf9 cell with cationic lipid reagent Cellfectin,homological recombination and plaque purification,the pure recombinant baculovirus clone named rBACTE2-339 were harvested and proliferated in sf9 cell to generate P2 stock.After infection with P2 stock,brilliant green fluorescence was detected under the fluorescence microscopy confirming the heterogenous protein expression of recombinant baculovirus in sf9 cell.