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Optimization of enzymatic hydrolysis conditions of pigskin.

Liang Wu

Open publisher page 1 citations

Abstract

Hydrolysis conditions of pigskin by Protamex were studied and optimized by single factor and Box-Benhnken response surface methodology(RSM)using hydrolysis degree as evaluation index. Results showed that the optimum conditions of enzymatic hydrolysis of pigskin were as the following: pH 6.3, temperature 53.2 ℃, enzyme dosage 2.65%, substrate concentration 15.0%, and hydrolysis time 6 hours, under which the hydrolysis degree was 16.13%.

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Hydrolysis conditions of pigskin by Protamex were studied and optimized by single factor and Box-Benhnken response surface methodology(RSM)using hydrolysis degree as evaluation index. Results showed that the optimum conditions of enzymatic hydrolysis of pigskin were as the following: pH 6.3, temperature 53.2 ℃, enzyme dosage 2.65%, substrate concentration 15.0%, and hydrolysis time 6 hours, under which the hydrolysis degree was 16.13%.

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Available abstract

Hydrolysis conditions of pigskin by Protamex were studied and optimized by single factor and Box-Benhnken response surface methodology(RSM)using hydrolysis degree as evaluation index. Results showed that the optimum conditions of enzymatic hydrolysis of pigskin were as the following: pH 6.3, temperature 53.2 ℃, enzyme dosage 2.65%, substrate concentration 15.0%, and hydrolysis time 6 hours, under which the hydrolysis degree was 16.13%.

Key concepts: Hydrolysis, Enzymatic hydrolysis, Chemistry, Response surface methodology, Chromatography, Enzyme, Substrate (aquarium), Degree (music)

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