RA Induced Apoptosis of Primary Cultured Craniopharyngiomic Cells in Vitro

Objective To explore the inducing effects of RA on the apotosis of primary cultured craniopharyngiomic cells.Methods Caniopharyngiomic cells were pimary cultured from fresh surgical samples,and then were treated with 20 mol RA for east 24 hours.Then these cells were collected for apoptotic tests which included Annexin V,DAPI staining and DNA ladder.Results RA treated primary cells presented higher level of Annexin V than untreated cells.Meanwhile DAPI staining indicated RA incubation leaded to pyknosis and nuclear condensation.In consistent,DNA ladder experiments were positive in RA treated primary cultured craniopharyngiomic cells.The results showed RA induced apoptosis of caniopharyngiomic cells.Conclusion 20 μmol of RA can successfully induce apoptosis of primary cultured craniopharyngiomic cells in vitro after 24 hours incubation,so as to kill craniopharyngiomic cells by initiating the apoptosis process.