Construction and expression of recombinant mouse interleukin 10 eukaryotic expression vector

Objective To construct an eukaryotic expression plasmid contained murine IL-10 gene and investigate the expression of the recombinant plasmid in 293T cells. Methods The gene of murine IL-10 was obtained by RT-PCR, and then was integrated into a eukaryotic expression plasmid pcDNA3.0, and finally the recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The plasmid was transfected into 293T cells by liposome transfecting technique and was identified by Western blot. Results The recombinant plasmid pcDNA3.0-mIL-10 was constructed successfully. Furthermore, the protein was extracted from 293T cells, transfected with the plasmid showed reactivity with antibody against IL-10. Conclusion The recombinant pcDNA3.0-mIL-10 was constructed successfully and was highly expressed in 293T cells, which will lay a foundation for the further study of the biologic activity of IL-10 in vivo.