Determination of palmatine hydrochloride and berberine hydrochloride in traditional Chinese patent medicine Qinlian tablets by HPLC

Objective To establish an HPLC method for simultaneous determination of palmatine hydrochloride and berberine hydrochloride in Qinlian tablets.Methods The separation was performed on a Diamonsil C18(4.6 mm ×200 mm,5 μm)column.The mobile phase was composed of 50 mmol·L-1 NaH2PO4(pH 4.6) and acetonitrile at the rate of V∶V=72∶28.The column temperature was set at 35 ℃ and the flow rate was 1.0 mL·min-1,with UV detection at 343 nm.Results The numbers of theoretical plates calculated by palmatine hydrochloride and berberine hydrochloride were 4 000 and 6 500,respectively.The regression equation of palmatine hydrochloride was A=3.706×104ρ+7.369×102(r=0.999 8),which was linear over the ranger of 5.2-52.0 mg·L-1.The regression equation of berberine hydrochloride was A=3.377×104ρ+8.978×103(r=0.999 7),which was linear over the ranger of 30.4-304.0 mg·L-1.The average recovery was 100.1 %(RSD=1.1 %) for palmatine hydrochloride,99.9%(RSD=0.80%) for berberine hydrochloride.Conclusions The method can be used in the quality control of Qinlian tablets.