Regeneration system development for different melon varieties

The influence of plant growth regulator combination,Ag NO3 concentration and genotype of melon on shoot organogenesis were investigated. The optimal concentrations of growth regulators were MS + 1.0 mg·L-16-BA + 0.5 mg·L-1ABA.The adventitious buds regeneration rates of‘IVF501',‘IVF509',‘IVF525',and‘IVF604'were 88.00%,79.60%,76.50%,and 74.75%,respectively. Different concentrations of Ag NO3 influenced melon adventitious bud regeneration. 1.0mg·L-1Ag NO3 effectively inhibited the callus formation and reduced the occurrence of the vitrification,the concentration of2.0 mg·L-1inhibited the callus formation,but at the same time reduced the rate of adventitious bud regeneration. The shoot elongation medium was MS + 0.05 mg·L- 16-BA. The rooting medium was MS + 0. 4 mg·L- 1IAA. It took about 50 to 60 days to obtain plantlet for‘IVF501'and 60 to 75 days for‘IVF525'from explants.