Study on the regeneration ability of six melon cultivars
Lu Lu
Abstract
Lu Lu
Abstract
In this paper, the regeneration ability of 6 melon cultivars was studied. The results showed that the adventitious buds of different melon genotypes were different on the same culture medium. In these melon cultivars, the regeneration rate of adventitious bud of Lubaoshi was the highest, reached 100%; followed by Xiyu-1 and Huanghemi, 96.67% and 73.33% respectively. The lower induction rates were of Yujinxiang and Huangzuixian, and the lowest was of Bailangua, only 6.67%. On the elongation medium, shoot buds were capable of elongating apparently. When the adventitious bud elongated to 2 cm, then transferred for root differentiation and at last the regenerated plantlet was formed on the rooting medium. Meanwhile, the effect of light on inducing adventitious buds was studied as well. The results showed that the illumination has effect on the rate of adventitious bud induction. The adventitious bud induction rates of 6 cultivars were decreased after the explants were pre-cultured in the dark for 5 days. However, the callus induction was increased or not influenced.
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In this paper, the regeneration ability of 6 melon cultivars was studied. The results showed that the adventitious buds of different melon genotypes were different on the same culture medium. In these melon cultivars, the regeneration rate of adventitious bud of Lubaoshi was the highest, reached 100%; followed by Xiyu-1 and Huanghemi, 96.67% and 73.33% respectively. The lower induction rates were of Yujinxiang and Huangzuixian, and the lowest was of Bailangua, only 6.67%. On the elongation medium, shoot buds were capable of elongating apparently. When the adventitious bud elongated to 2 cm, then transferred for root differentiation and at last the regenerated plantlet was formed on the rooting medium. Meanwhile, the effect of light on inducing adventitious buds was studied as well. The results showed that the illumination has effect on the rate of adventitious bud induction. The adventitious bud induction rates of 6 cultivars were decreased after the explants were pre-cultured in the dark for 5 days. However, the callus induction was increased or not influenced.
Key concepts: Melon, Plantlet, Cultivar, Basal shoot, Biology, Callus, Regeneration (biology), Explant culture