Methods of Zoospore Inducing and Preservation for Phytophthora capsici

[objective] Researches on methods of zoospore inducing and preservation for Phytophthora capsic.were carried out.[methed]The strain was cultured in five different media for 3 d with darkness,then for 6 d with illumination at 26 ℃.Every medium was divideded into 2 group adding 40 ml sterile water respectivetly.One was used to calculate sporangium yield,the other was lighted 0.5 h at 16 ℃,then zoospores were calculated.[Result] Medium types affected P.capsici colony shape.The colony grow speed was OMACMACAPSABA.There were the most sporangia in BA and PSA medium and the least in CMA medium.But the most zoospore yeild in CMA medium.There were no significant difference in BA、PSA、CA and OMA medium.The strain can be preserved for 9 months in test-tube.[Conclusion] It is the best method for inducing zoospore that the strain was cultured in CMA medium for 3 d with darkness,then for 6 d with illumination at 26 ℃.Optimum preservation temperature is 16 ℃;There was no correlation between zoospore and sporangium yeild in different medium.