ScholarLens Back to search
2002•Yiyao daobaoRequires access

Determination of Extract Ginkgo Biloba in Blood Plasma of Rabbits

Yang Qi

Open publisher page 1 citations

Abstract

Objective:To establish the method of determining extract ginkgo biloba(EGB) in blood plasma of rabbits by RP HPLC and study the pharmacokinetics of EGB in rabbits. Method:Hypersil column (100 mm×2.1 mm, 5 μm) of the analytical column was ODS. The mobile phase consisted of methanol water phosphate (32∶67.6∶0.4). The flow rate was 1.0 mL·min 1 . The UV detection was at 368 nm. Results:The lower limit of determination was 1.25 μg·mL 1 . The average recovery of the method was 98.82% 107.80%. The related standard deviation ( RSD ) was 4%( n =3). The linear limits of high and low blood concentrations ranged from 2.5 5.00 μg·mL 1 ( r =0.997 2, n =5)and 6.25 15.00 μg·mL 1 ( r =0.995 7, n =5) respectively. Conclusion:The method of determination for EGB in the blood plasma of rabbits by RP HPLC is established in this experiment. It has the advantages of being easy to operate, accurate and sensitive.

About this research paper

What this paper is about

Objective:To establish the method of determining extract ginkgo biloba(EGB) in blood plasma of rabbits by RP HPLC and study the pharmacokinetics of EGB in rabbits. Method:Hypersil column (100 mm×2.1 mm, 5 μm) of the analytical column was ODS. The mobile phase consisted of methanol water phosphate (32∶67.6∶0.4). The flow rate was 1.0 mL·min 1 . The UV detection was at 368 nm. Results:The lower limit of determination was 1.25 μg·mL 1 . The average recovery of the method was 98.82% 107.80%. The related standard deviation ( RSD ) was 4%( n =3). The linear limits of high and low blood concentrations ranged from 2.5 5.00 μg·mL 1 ( r =0.997 2, n =5)and 6.25 15.00 μg·mL 1 ( r =0.995 7, n =5) respectively. Conclusion:The method of determination for EGB in the blood plasma of rabbits by RP HPLC is established in this experiment. It has the advantages of being easy to operate, accurate and sensitive.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish the method of determining extract ginkgo biloba(EGB) in blood plasma of rabbits by RP HPLC and study the pharmacokinetics of EGB in rabbits. Method:Hypersil column (100 mm×2.1 mm, 5 μm) of the analytical column was ODS. The mobile phase consisted of methanol water phosphate (32∶67.6∶0.4). The flow rate was 1.0 mL·min 1 . The UV detection was at 368 nm. Results:The lower limit of determination was 1.25 μg·mL 1 . The average recovery of the method was 98.82% 107.80%. The related standard deviation ( RSD ) was 4%( n =3). The linear limits of high and low blood concentrations ranged from 2.5 5.00 μg·mL 1 ( r =0.997 2, n =5)and 6.25 15.00 μg·mL 1 ( r =0.995 7, n =5) respectively. Conclusion:The method of determination for EGB in the blood plasma of rabbits by RP HPLC is established in this experiment. It has the advantages of being easy to operate, accurate and sensitive.

Key concepts: Ginkgo biloba, Chromatography, Chemistry, High-performance liquid chromatography, Pharmacokinetics, Detection limit, Ginkgo, Pharmacology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Extract Ginkgo Biloba in Blood Plasma of Rabbits — Research Paper | ScholarLens