Effects of Vitrification on the Development of Mouse GV-oocytes

To investigate the potential development of thawed GV-oocytes,the mouse GV-occytes(without comulus cells) were vitrified using OPS method and the mouse ovary was vitrified by straw method employing EDFS30 as vitrification solution for the first time.At first,in order to choose the optimal culture system for GV-oocytes after vitrification,the fresh GV-oocytes were cultured in MEM or MEM-preantral follicle.The result showed that there was no significant difference between the two culture methods;the rates of maturation or the rates of cleavage did not differ between the fresh and vitrified group(P 0.05).However,the GV-oocytes separated from the vitrified ovary showed significant difference(P 0.01) with the control in the rates of maturation,and no zygote was gained in the present study.In Conclusion,GV-oocytes can be cryopreserved efficiently by OPS method,while the GV-oocytes derived from vitrified ovary showed limited potential viability.