Studies on cryopreservation of mouse oocytes

The anti-freezing competences of mouse GV oocytes cryopreserved by procedure and vitrification were studied after thawing. PBS was used as holding mediums and Glycerol and DMSO were used as cryopretectants. The results showed that the method of cryopreservation did not have significant effects on the rate of natural and survival mouse oocytes (P0.05). The cryoprotectants used in vitrification freezing did not have significant effect on the rate of natural mouse oocytes (P0.05) but on the rate of survival mouse oocytes, and DMSO was very significantly better than Glycerol in vitrification freezing (P0.01). The anti-freezing competences of mouse GV and MⅡoocytes cryopreserved by vitrification were evaluated, which showed better results when using cryopretectant DMSO. There was no significant difference on the rate of nature between GV and MⅡoocytes (P0.05), but the rate of survival mouse GV oocytes was significantly better than that of MⅡoocytes (P0.05).