Inhibitory effect of ginsenoside Rb1 on busulfan-induced apoptosis in human umbilical vein endothelial cells

Objective:To observe the busulfan-induced apoptosis in human umbilical vein endothelial cells(HUVEC)and the effect of ginsenoside Rb1 on this type of apoptosis.Method:HUVEC were cultivated and divided into several groups randomly,which were administrated with busulfan at different concentrations for 12 or 24 hours to determine appropriate busulfan treatment dosage and duration.Then HUVECs were pre-incubated with ginsenoside Rb1 and subsequently exposed to busulfan.Apoptosis was detected by Flow Cytometry using Annexin-V assay.Result:Busulfan at high concentration(0.12 g/L)promoted the ratio of the endothelial cells apoptosis.This apoptosis inducing effect was inhibited by ginsenoside Rb1,significantly distinctive in different dosage groups.Conclusion:Busulfan at high concentration could initiate apoptosis in cultivated HUVEC,which could induce endothelium damage in hematopoietic stem cell transplantation.Ginsenoside Rb1 might protect endothelial cells by inhibiting busulfan-induced apoptosis.