Effect of ginsenoside on endothelial cells apoptosis induced by hypoxia-reoxygenation

Objective:To observe the endothelial cells apoptosis induced by hypoxia and reoxygenation and the inhibiting effect of ginsenoside Rb1.Method:Human umbilical vein endothelial cells(HUVECs) were cultivated and divided into five groups randomly,which were used to establish the hypoxia-reoxygenation models.TUNEL technology was used to quantify the apoptotic and necrotic cells.DNA electrophoresis was utilized to determine changes characteristic of apoptosis.Result:Percentage of apoptosis in different groups was significantly distinctive,which increased steadily with the duration of hypoxia-reoxygenation.apoptic cells in Rb1 group were significantly less than in the hypoxia-reoxygenation group(P0.05).Conclusion:Hypoxia-reoxygenation could initiate apoptosis in cultivated HUVECs in a time-dependent manner.Rb1 exerts cell protection in hypoxia-reoxygenation injury by inhibiting endothelial cells apoptosis.