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Effect of propolis water extract on apoptosis of human umbilical vein endothelial cells in vitro

Bin Chen

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Abstract

AIM:To explore the effect of water extract propolis(WEP)from Taishan on apoptosis of human umbilical vascular endothelial cells(HUVECs)induced in vitro by tumor necrosis factor-α(TNF-α).METHODS:HUVECs were collect by digestion-perfusion and cultivated.TNF-α at concentration of 50 μg/L was administrated to induce the apoptosis of HUVECs.After injury,HUVECs were treated with WEP at concentrations of 50,100,and 200 mg/L,respectively,for 24 h.Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and flow cytometry(FCM).RESULTS:Apoptosis index in injured group was significantly higher than that in control group,and decreased significantly after treating with WEP at concentrations of 50,100,and 200 mg/L,respectively(P0.01).CONCLUSION:WEP may be useful for protection of vascular endothelial cells by inhibiting apoptosis.

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AIM:To explore the effect of water extract propolis(WEP)from Taishan on apoptosis of human umbilical vascular endothelial cells(HUVECs)induced in vitro by tumor necrosis factor-α(TNF-α).METHODS:HUVECs were collect by digestion-perfusion and cultivated.TNF-α at concentration of 50 μg/L was administrated to induce the apoptosis of HUVECs.After injury,HUVECs were treated with WEP at concentrations of 50,100,and 200 mg/L,respectively,for 24 h.Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and flow cytometry(FCM).RESULTS:Apoptosis index in injured group was significantly higher than that in control group,and decreased significantly after treating with WEP at concentrations of 50,100,and 200 mg/L,respectively(P0.01).CONCLUSION:WEP may be useful for protection of vascular endothelial cells by inhibiting apoptosis.

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Available abstract

AIM:To explore the effect of water extract propolis(WEP)from Taishan on apoptosis of human umbilical vascular endothelial cells(HUVECs)induced in vitro by tumor necrosis factor-α(TNF-α).METHODS:HUVECs were collect by digestion-perfusion and cultivated.TNF-α at concentration of 50 μg/L was administrated to induce the apoptosis of HUVECs.After injury,HUVECs were treated with WEP at concentrations of 50,100,and 200 mg/L,respectively,for 24 h.Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and flow cytometry(FCM).RESULTS:Apoptosis index in injured group was significantly higher than that in control group,and decreased significantly after treating with WEP at concentrations of 50,100,and 200 mg/L,respectively(P0.01).CONCLUSION:WEP may be useful for protection of vascular endothelial cells by inhibiting apoptosis.

Key concepts: Umbilical vein, TUNEL assay, Apoptosis, Propolis, Flow cytometry, In vitro, Tumor necrosis factor alpha, Terminal deoxynucleotidyl transferase

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