Separation and Purification and Constituent Structural Analysis of Foshou Yam Polysaccharide

Objective To analyse Foshou yam polysaccharide and its constituent structural.METHODS Foshou yam as raw material,using the extraction method of water extract-alcohol precipitation,through a series of separation and purification steps get polysaccharide components.Use the UV,IR,TLC and other modern instrument analysis methods to analyze polysaccharide structure.CONCLUSION two neutral polysaccharide components(YPa-Ⅰ and YPa-Ⅱ) and an acidic polysaccharide(YPbs) are obtained.UV analysis shows that three types of polysaccharide are not contains proteins,nucleic acids and polypeptides,and IR scanning proves that three kinds of polysaccharide have the characteristic absorption peak of polysaccharide.TLC analysis of the monosaccharide composition of three kinds of polysaccharide shows that the neutral polysaccharide YPa-Ⅰ’s monosaccharide composition is arabinose,fructose and glucose.The neutral polysaccharide YPa-Ⅱ’s monosaccharide composition is fructose,mannose and glucose,the acidic polysaccharide YPbs’s monosaccharide composition is xylose,galactose and glucose.With multi-angle laser light scattering detector and refractive index detector,gel permeation chromatography determined molecular weight of yam polysaccharide,the yam neutral polysaccharide components of YPa-Ⅰ,YPa-Ⅱ average molecular weights are 65086 and 20982 respectively.The yam acidic polysaccharide component YPbs average molecular weight is 34780.