Study on Key Technique of Rapid Propagation of Phalaenopsis amabilis

Picking tender leaves,shoot-tips,pedicel lateral bud,pedicel internode as explants to induce original corm,to search the best explants on tissue culturing of Phalaenopsis amabilis;Different culture medium with hormone combination were screened to original corm induction,proliferation and rooting of seedling in test tube;Searching the optimal medium in different culturing stage.The result showed:the shoot-tips and pedicel lateral buds are the best explants for inducing original corm;The best culture medium of proliferation for original corm is MS+0.5 mg/L NAA+1.0 mg/L 6-BA+100 ml/L coconut milk,proliferation ratio is 8.98;1/2MS culture medium is the best to induce lateral bud forming clump buds;The optimum culture medium for rooting is MS +1.0 mg/L IBA +0.5 mg/L NAA.