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Expression of porcine Toll-like receptor TLR2 gene in the CHO-K1 cells

Yongxi Dou

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Abstract

The recombinant plasmid PcDNA3.1/CT-GFP-pTLR2 was transfected into the CHO-K1 cells by lipofectin and the positive cell clone was obtained after screening by G418 for 2~3 weeks.Analysis of the exogenous gene by PCR and RT-PCR indicated that the pTLR2 gene was integrated into the CHO-K1 cells.The expression of pTLR2-GFP fusion protein was observed by intensity fluorescence microscope at different time after transfection.The results indicated that pTLR2-GFP fusion protein was successfully expressed in the cells and these cells could be further used to investigate the functions of pTLR2.

About this research paper

What this paper is about

The recombinant plasmid PcDNA3.1/CT-GFP-pTLR2 was transfected into the CHO-K1 cells by lipofectin and the positive cell clone was obtained after screening by G418 for 2~3 weeks.Analysis of the exogenous gene by PCR and RT-PCR indicated that the pTLR2 gene was integrated into the CHO-K1 cells.The expression of pTLR2-GFP fusion protein was observed by intensity fluorescence microscope at different time after transfection.The results indicated that pTLR2-GFP fusion protein was successfully expressed in the cells and these cells could be further used to investigate the functions of pTLR2.

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Available abstract

The recombinant plasmid PcDNA3.1/CT-GFP-pTLR2 was transfected into the CHO-K1 cells by lipofectin and the positive cell clone was obtained after screening by G418 for 2~3 weeks.Analysis of the exogenous gene by PCR and RT-PCR indicated that the pTLR2 gene was integrated into the CHO-K1 cells.The expression of pTLR2-GFP fusion protein was observed by intensity fluorescence microscope at different time after transfection.The results indicated that pTLR2-GFP fusion protein was successfully expressed in the cells and these cells could be further used to investigate the functions of pTLR2.

Key concepts: Transfection, Chinese hamster ovary cell, Molecular biology, Green fluorescent protein, Recombinant DNA, Fluorescence microscope, clone (Java method), Gene

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