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2004•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access

Transfection expression of VEGF_(121) in mammalian cells

Jinfu Yang

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Abstract

Objective: To investigate the transfection and expression of VEGF121 in mammalian cells. Methods: pcDNA3.1-VEGF121 plasmid were transfected into CHO cells by electroporation and clones were screened by G418. The transcription and expression of VEGF121 in CHO cells were tested by RT-PCR, ELISA and Western-blot. The biologic activities of VEGF121 secreted by CHO cells were investigated by Mile′s assay and endothelial cells MTT assay. Results: We Obtained anti-G418 CHO cells mono-clones. RT-PCR, ELISA and Western-blot proved that there was transcription and expression of VEGF121 in anti-G418 CHO cells. The expressed VEGF121 could increase the vessel permeability and promote the proliferance of endothelial cells. Conclusions: pcDNA3.1-VEGF121 plasmid can express active VEGF121 well in mammalian cells.

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What this paper is about

Objective: To investigate the transfection and expression of VEGF121 in mammalian cells. Methods: pcDNA3.1-VEGF121 plasmid were transfected into CHO cells by electroporation and clones were screened by G418. The transcription and expression of VEGF121 in CHO cells were tested by RT-PCR, ELISA and Western-blot. The biologic activities of VEGF121 secreted by CHO cells were investigated by Mile′s assay and endothelial cells MTT assay. Results: We Obtained anti-G418 CHO cells mono-clones. RT-PCR, ELISA and Western-blot proved that there was transcription and expression of VEGF121 in anti-G418 CHO cells. The expressed VEGF121 could increase the vessel permeability and promote the proliferance of endothelial cells. Conclusions: pcDNA3.1-VEGF121 plasmid can express active VEGF121 well in mammalian cells.

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Available abstract

Objective: To investigate the transfection and expression of VEGF121 in mammalian cells. Methods: pcDNA3.1-VEGF121 plasmid were transfected into CHO cells by electroporation and clones were screened by G418. The transcription and expression of VEGF121 in CHO cells were tested by RT-PCR, ELISA and Western-blot. The biologic activities of VEGF121 secreted by CHO cells were investigated by Mile′s assay and endothelial cells MTT assay. Results: We Obtained anti-G418 CHO cells mono-clones. RT-PCR, ELISA and Western-blot proved that there was transcription and expression of VEGF121 in anti-G418 CHO cells. The expressed VEGF121 could increase the vessel permeability and promote the proliferance of endothelial cells. Conclusions: pcDNA3.1-VEGF121 plasmid can express active VEGF121 well in mammalian cells.

Key concepts: Transfection, Electroporation, Molecular biology, Western blot, Biology, Titer, Chemistry, Cell culture

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