Expression and bioactive analysis of porcine interleukin 4 in the CHO-K1 cells

The IL-4 gene of porcine was subcloned into the eukaryotic expression vector pEGFP-N1 to construct the recombinant plasmid pEGFP-PIL-4,which was transfected into the CHO-K1 cells by the method of using Lipofectin,The expression of PIL-4 was determined by fluoroscopy,RT-PCR and Western-blot.After 24 hours and 48 hours,the Green fluorescence can be observed under fluorescence microscope and a 339 bp cDNA was detected by RT-PCR after screening 14 days with G418,39 000 protein was detected by Western-blot.The results indicate that fusion protein of PoIL-4 and EGFP was successfully expressed in CHO-K1 cells.And expressed protein had obviously biological activity.