Effects of Fosinopril on Expression of Transforming Growth Factor β1 of Rat Glomerular Mesangial Cell

Objective To observe effects of fosinopril (FOS) on proliferation and expression of transforming growth factor(TGF) β1 of rat glomerular mesangial cell (GMC) induced by lipopolysaccharide(LPS). Methods After establishing the cultured glomerular mesangial cells of rat in vitro, passages 3~10 of cells were used in the experiment after identification. The experiment included 5 groups: control group(Ctrl group), lipopolysaccharide group(LPS group), high dose fosinopril group(FOS 1 group), middle dose fosinopril group(FOS 2 group) and low dose fosinopril group(FOS 3 group). Glomerular mesangial cells proliferation were detected by methyl thiazolyl tetrazolium (MTT) incorporation method at 24 h and 48 h, and the change of transforming growth factor β1 protein secretion was detected by the enzyme-linked immunosorbent-assay (ELISA). The change of transforming growth factor β1 mRNA expression was detected by semi-quantitative real-time(RT)-PCR. Results Lipopolysaccharide can induce the glomerular mesangial cell proliferation, fosinopril can inhibit the effect of proliferation induced by lipopolysaccharide. Gomerular mesangial cells had transforming growth factor β1 protein secretion and mRNA expression at certain level in normal culture medium, transforming growth factor β1 protein secretion and mRNA expression were higher significantly in LPS group than those in Ctrl group (P<0.01), but lower significantly in all of fosinopril groups than those in LPS group (P<0.01). Conclusion Fosinopril can inhibit the effect of proliferation and the expression of transforming growth factor β1 in glomerular mesangial cells at the mRNA and protein levels induced by lipopolysaccharide, and provides the theoretical evidence for the renal protection of fosinopril. Key words: glomerular mesangial cell; transforming growth factor β1; fosinopril