Determination of Polysaccharide in Seabuckthorn Leaves by Spectrophotometry
Zhao Er
Abstract
Zhao Er
Abstract
The polysaccharide in seabuckthorn leaves was determined with phenol-sulfuric acid at 490nm by spectrophotometry.The linear range is of 10.0—71.0μg/mL with r=0.9981.The conversion factor(f) is 3.271.The contents of polysaccharide of seabuckthorn leaves in different seasons at same place are:3.39% in May,3.94% in July and 4.42% in September respectively.The average recovery is 99.07% with RSD of 3.22%.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
The polysaccharide in seabuckthorn leaves was determined with phenol-sulfuric acid at 490nm by spectrophotometry.The linear range is of 10.0—71.0μg/mL with r=0.9981.The conversion factor(f) is 3.271.The contents of polysaccharide of seabuckthorn leaves in different seasons at same place are:3.39% in May,3.94% in July and 4.42% in September respectively.The average recovery is 99.07% with RSD of 3.22%.
Key concepts: Polysaccharide, Spectrophotometry, Chemistry, Phenol, Chromatography, Linear relationship, Linear range, Sulfuric acid